Research Use Serum Melatonin EIA Kit

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Direct Serum Melatonin Enzyme Immunoassay

Quantitative measurement of Melatonin in human serum or plasma.

RUO

534

Enzyme Immunoassay (EIA), Colormetric

3.0 – 300 pg/mL

2.5 pg/mL

25 µL

2hrs @ Room Temperature

96 wells

450 nm

2° - 8°C

6 Months

Summary and Assay Principle:

Melatonin (N-Acetyl-5-methoxytrptamine) is a biogenic amine that is found in animals and plants. In mammals, melatonin is produced by the pineal gland. Its secretion increases in darkness and decreases during exposure to light. Melatonin is implicated in the regulation of sleep, mood and reproduction. Melatonin is also an effective antioxidant. (1-6)

Assay Principles:

The Pantex Direct Melatonin Serum/Plasma Kit, Cat #534 is based on the competition principal and microplate separation. Melatonin in calibrators and samples compete with a fixed amount of melatonin conjugated to horse radish peroxidase (Melatonin-HRP) for binding sites with a rabbit melatonin monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of Melatonin-HRP detected is inversely proportional to the amount of melatonin in a sample.