Research Use Salivary Free Estriol(uE3) EIA

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Salivary Unconjugated (Free) Estriol Enzyme Immunoassay

Quantitative measurement of salivary Free Estriol.

RUO

633

Enzyme Immunoassay (EIA), Colormetric

5.0 pg/mL–1,215 pg/mL

0.8 pg/mL

50 µL

2hrs @ Room Temperature

96 wells

450 nm

2° - 8°C

12 Months

Summary and Assay Principle:

Estriol is the weakest of the three major estrogens with a binding affinity for the estrogen receptor about 1/100 that of estradiol. Physiologically significant amounts of Estriol are only present during pregnancy or with supplementation. Estriol is available, mainly, as a custom compounded product and is one of the components of bi- or tri-estrogen creams usually in a ratio of estradiol to estrone to estriol 1:1:8. Salivary estriol median levels are <2 pg/ml in non- pregnant pre- and post-menopausal women as well as in men. These levels represent little to no physiologic estrogenic activity. In pregnancy, low levels of Estriol may indicate fetal distress and in Down Syndrome Estriol measurements are part of a screen for this condition.

Assay Principles:

The Pantex Salivary Free Estriol EIA kit, Cat #633, is based on the competition principal and microplate separation. Estriol calibrators and unknown amounts of estriol in saliva samples compete with a fixed amount of estriol conjugated to horse radish peroxidase (Estriol-HRP) for binding sites with a rabbit estriol monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of Estriol-HRP detected is inversely proportional to the amount of estriol in a sample.